Figure 4. Validation of circRNA expression in neonatal pig hearts.

(A–J) qRT-PCR was performed to validate the expression of 10 selected circRNAs associated with the cell cycle regulation pathway in neonatal pig hearts. All data were presented as mean ± SEM. Statistical comparisons between 2 groups in multiple groups were evaluated by 1-way ANOVA with Tukey’s honestly significant difference test for panels A–J. n = 6 pig hearts for each group. *P < 0.05, **P < 0.01, ***P < 0.001, and ****P < 0.0001 when comparing the expression levels between them. The relative expression levels of circRNAs were normalized to GAPDH reference gene.