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. 2024 Jun 25;9(15):e175625. doi: 10.1172/jci.insight.175625

Figure 8. Impact of circRNA knockdown on the expression of miRNAs.

Figure 8

hiPSC-CMs at day 28 after the initiation of cardiac differentiation were used. qRT-PCR was used to evaluate the expression level of various miRNAs in hiPSC-CMs. This included the evaluation of hsa-miR-31-5P, hsa-miR-197-3P, hsa-miR-215-3P, hsa-miR-214-5P, and hsa-miR-105-3P in hiPSC-CMs treated with hsa-ABLIM1_0001 siRNA (A), hsa-miR-140-5P and hsa-miR-15a-3P in hiPSC-CMs treated with hsa-KIF1B_0001 siRNA (B), and hsa-miR-128-3P in hiPSC-CMs treated with hsa-AC096949_0001 siRNA (C). The relative expression levels of miRNAs were normalized to U6 reference gene. All data were presented as mean ± SEM. Statistical analysis was performed via Student’s 2-tailed t test. n = 3 technical replicates in each group. *P < 0.05, **P < 0.01, and ***P < 0.001.