Figure 8.

The figure shows sections that are rostral to the locus coeruleus (LC) central core. The final section of the LC rostral horn is shown in the section marked 1250. Subsequent sections are beyond the boundary of the LC. Note that these sections still, however, contain tyrosine hydroxylase (TH)-positive neurons, such as subcoeruleus (SubC)-norepinephrine (NE) neurons. The immunostained (dark brown) neurons are in the dorsal brainstem (SubC). The images are serial coronal sections of the rat (male) brainstem in 50-μm thickness continuing from Figs. 6 and 7. All sections are the same scale (see 100 μm scale bar). Upward is dorsal and leftward is lateral. The sections alternate Nissl (violet) and TH immunostain (dark brown). The top left section is labeled in μm from the most caudal section in the upper left corner of Fig. 6. Subsequent serial sections are labeled in 50-μm increments and continue rostral (through Fig. 9).