Figure 7. Inhibitory pre- and post-synaptic markers are relatively normal in Calb1^Cre;Dag1^cΔICD cerebellar cortex.

(A-B) Tissue sections from cerebellar vermis of Calb1^Cre;Dag1^cΔICDs (B) and littermate controls (A) was immunostained for Calbindin to visualize Purkinje cells along with the presynaptic marker VGAT (magenta) and postsynaptic GABA_A receptor subunit GABA_Aα1 (green). The first panel shows a low-magnification view of the Calbindin channel. Subsequent panels are magnified views of the yellow outlined region in the first panel. Images are maximum projections. (C) Quantification of VGAT (left) and GABA_Aα1 (right) puncta density (per 10μm³). Open points indicate statistical outliers, however no datapoints were excluded from statistical analysis. (D) Quantification of VGAT (left) and GABA_Aα1 (right) puncta size (in μm³). (E) Quantification of the distribution of VGAT (left) and GABA_Aα1 (right) puncta sizes (in μm³), normalized to a maximum of 1. Calb1^Cre;Dag1^WT N = 13 images, 3 animals. Calb1^Cre;Dag1^cKO N = 13 images, 3 animals.