Table 1 –

Mutations targeted for correction in C321. K-12 lineage mutations are present in all K-12 derived strains, and C321 lineage mutations are unique to C321 and its derivatives.

Site	Mutation	Lineage	Function
ilvG	∆(A979-T980)	K-12	Isoleucine biosynthesis
prfB	Ala246Thr	K-12	UGA/UAA release factor
gpp	Arg134His	C321	Stringent response
folA promoter	promoter −35 box	C321	Tetrahydrofolate biosynthesis
cyaA	Pro301Leu	C321	cAMP biosynthesis and signaling
leuS	Val613Phe	C321	Leucine aminoacyl tRNA synthetase
bamA	Pro763Ser	C321	Outer membrane protein assembly
hemA	Leu196Pro	C321	Porphyrin biosynthesis
cpxA	Trp184Arg	C321	Inner membrane stress response