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. 2024 Aug 9;5(3):103240. doi: 10.1016/j.xpro.2024.103240

Figure 1.

Figure 1

Workflow for expression of recombinant VDAC1 in E. coli

Expression starts with inoculation of an “overnight culture” with a single colony of E. coli carrying a 6xHis-mVDAC1 or 6xHis-hVDAC1 expression plasmid. After induction with IPTG, cells are harvested and can be stored at −20°C.