Figure 8.

Liposome size determined by dynamic light scattering

10 μL extruded liposomes were diluted into 600 μL Activity Assay Buffer in a 1.5 mL plastic cuvette. Three independent measurements were taken on the same sample at 25°C using preset parameters for phospholipids as the material (refractive index = 1.4500, absorption index = 0.0010) and 154 mM NaCl as the solvent (refractive index = 1.3318254, viscosity = 0.000906425 Pa s, relative permittivity = 76.59715402). Error bars = mean ± SD (n = 3). The solid line corresponds to Gaussian least squares fit indicating a mean diameter (± SD) of 172 ± 42 nm.