Table 1.

Fractions to save for SDS-PAGE analysis of protein purification

	Name	Volume loaded	Description of sample (total volume)	Step in protocol
1	Uninduced	See step 5	Total protein content of culture before inducing expression of mVDAC1 with IPTG (1 L).	5
2	Induced	See step 9	Total protein content of culture after inducing expression of mVDAC1 with IPTG (1 L).	9
3	Supernatant	3 μL	Supernatant of centrifuged cell lysate (30 mL). Should contain very little VDAC1, as most of the protein was pelleted as inclusion bodies.	13.f
4	Load	3 μL	Total soluble material to be loaded on Ni-NTA column (30 mL).	13.m
5	Flow through	3 uL	Material that did not bind to the Ni-NTA resin (30 mL).	14.f
6	Wash	3 μL	Material that was removed from Ni-NTA resin by washing with 20 mM imidazole (50 mL).	14.h
7	Elution 1	3 μL	First fraction eluted from Ni-NTA resin with 150 mM imidazole (5 mL).	14.i
8	Elution 2	3 uL	Second fraction eluted from Ni-NTA resin with 150 mM imidazole (5 mL).	14.i
9	Elution 3	3 μL	Third fraction eluted from Ni-NTA resin with 150 mM imidazole (5 mL).	14.i