Activity Assay Buffer

Reagent	Final concentration	Amount
HEPES-NaOH, pH 7.4 (500 mM)	50 mM	5 mL
NaCl (5 M)	150 mM	1.5 mL
ddH2O	N/A	43.5 mL
Total	N/A	50 mL

Prepare from filtered or autoclaved stock solutions. Store at 20°C–24°C.