Resuspension Buffer

Reagent	Final concentration	Amount
Tris-HCl, pH 8.0 (1 M)	50 mM	25 mL
EDTA, pH 8.0 (0.5 M)	2 mM	2 mL
Sucrose	20% (w/v)	100 g
ddH2O	N/A	Bring up to 500 mL
Total	N/A	500 mL

Filter sterilize. Do not autoclave solutions containing sucrose. Store at 4°C for several months.