Inclusion Bodies Wash Buffer

Reagent	Final concentration	Amount
Tris-HCl, pH 8.0 (1 M)	20 mM	10 mL
NaCl (5 M)	300 mM	30 mL
CaCl2 (1 M)	2 mM	1 mL
ddH2O	N/A	Bring up to 500 mL
Total	N/A	500 mL

Filter sterilize or autoclave. Store at 20°C–24°C for immediate use or 4°C for several months.