Rapid Dilution Refolding Buffer

Reagent	Final concentration	Amount
Tris-HCl, pH 8.0 (1 M)	20 mM	0.2 mL
NaCl (5 M)	150 mM	0.3 mL
LDAO (30%, w/v)	1.5% (w/v)	0.5 mL
DTT (1 M)	10 mM	0.1 mL
ddH2O	N/A	7.9 mL
Total	N/A	9 mL

Prepare fresh buffer. Cool to 4°C before use.

Final concentrations are calculated for a 10 mL final volume, after the addition of 1 mL purified VDAC1 in step 18.