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Dialysis Buffer
| Reagent | Final concentration | Amount |
|---|---|---|
| Tris-HCl, pH 8.0 (1 M) | 20 mM | 20 mL |
| NaCl (5 M) | 150 mM | 30 mL |
| DTT (1 M) | 1 mM | 1 mL |
| ddH2O | N/A | 949 mL |
| Total | N/A | 1 L |
Filter sterilize or autoclave before adding DTT. Buffer can be stored at 4°C for several months without DTT. Add DTT just before use in step 21. Cool to 4°C before use.
