Reconstitution Buffer

Reagent	Final concentration	Amount
MOPS-KOH, pH 7 (100 mM)	10 mM	5 mL
KCl (1 M)	100 mM	5 mL
ddH2O	N/A	40 mL
Total	N/A	50 mL

Prepare from filtered or autoclaved stock solutions. Store at 20°C–24°C.