Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2024 Sep 9;15:7873. doi: 10.1038/s41467-024-52284-8

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© The Author(s) 2024

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

PMC Copyright notice

Fig. 2 — a Immunofluorescence for FLAG (Merlin) or streptavidin in M10G meningioma cells expressing Merlin^FLAG-APEX2 constructs after proximity-labelling. Representative of five biological replicates. Scale bar, 10 μm. b Heatmap of β-catenin biotinylation peptide intensity from proximity-labeling proteomic mass spectrometry as in (a) (n = 3/condition). c TOP-Flash Tcf/Lef luciferase reporter assay in M10G^dCas9-KRAB meningioma cells expressing sgRNAs suppressing NF2 (sgNF2) with or without rescue of Merlin^FLAG-APEX2 wildtype or cancer-associated missense constructs, n = 3. d Immunoblots for FLAG (Merlin) or β-catenin after biochemical fractionation of CH-157MN meningioma cells expressing Merlin^FLAG-APEX2 rescue constructs. Immunoblots for calreticulin, α-tubulin, vimentin, Rb, or histone H3 mark membrane (M), cytoplasmic (Cp), cytoskeletal (Cs), nuclear (N), or chromatin fractions (Ch), respectively. Representative of six biological replicates. e TOP-Flash Tcf/Lef luciferase reporter assay in M10G meningioma cells expressing non-targeted control siRNAs (siNTC) or siRNAs suppressing β-catenin (siCTNNB1) with or without 24 h of Wnt3a treatment (100 ng/μl). Data are representative of four biological replicates. f–h TOP-Flash Tcf/Lef luciferase reporter assays in M10G^dCas9-KRAB meningioma cells expressing non-targeted control sgRNAs (sgNTC) or sgNF2 with or without 24 h of Wnt3a treatment (100 ng/μl) in the presence or absence of β-catenin or Merlin overexpression or rescue. Data are representative of four biological replicates. f shows β-catenin overexpression fails to hyperactivate the Wnt pathway in the absence of Merlin. Data are representative of four biological replicates. g, h show Wnt stimulation is necessary for Merlin overexpression to hyperactivate the Wnt pathway. Data are representative of four biological replicates. Lines represent means, and error bars represent standard error of the means. **P ≤ 0.01, ***P ≤ 0.0001 (Student’s t test, one sided). Source data are provided as a Source Data file.