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. 2024 Sep 9;15:7863. doi: 10.1038/s41467-024-51353-2

Fig. 4. Mo/MΦ display a pro-healing transcriptomic profile upon Treg delivery.

Fig. 4

a Wildtype C57BL6/J mice with bone, muscle or skin injuries were treated with fibrin hydrogel only or hydrogel containing exogenous Tregs. b–f Endogenous Mo/MΦ from injured tissues were sorted for RNA sequencing on D4 and D7 post-injury. Heat maps depicting standardised gene expression values of selected significantly upregulated and downregulated genes (FDR adjusted p value < 0.05) in Mo/MΦ from Treg-treated bone (b), muscle (c), and skin (d) injuries, compared to untreated controls (n = 3 mice/tissue per time point; individual replicates are shown). Colour key denotes the functional category of the genes. Gene ontology terms depicting enriched biological processes in significantly upregulated (e) and downregulated (f) genes in Treg-treated tissues, from both D4 and D7 macrophages combined (FDR < 0.01, adjusted by Benjamini–Hochberg correction). (Mo/MΦ: monocytes/ macrophages). a Created with BioRender.com released under a Creative Commons Attribution-NonCommercial-NoDerivs 4.0 International license (https://creativecommons.org/licenses/by-nc-nd/4.0/deed.en).