Figure 4. p53^R172H occupies and modulates Cxcl1 enhancers.

A, p53^R172H occupancy at and around the Cxcl1 gene in Trp53^R172H/− and Trp53^−/− cells using CUT&RUN. Non-specific IgG in Trp53^R172H/− cells is used as a control.

B, p300, H3K27Ac, and H3K4me3 occupancy at and around the Cxcl1 gene in Trp53^R172H/− and Trp53^−/− cells using CUT&RUN.

C, Nascent RNA profiles at and around the Cxcl1 gene in Trp53^R172H/−, Trp53^−/−, or Trp53^−/− + pTrp53^R172H cells using PRO-seq. Nascent RNA profiles are used to call de novo enhancers using dREG⁷¹, and the annotated enhancers are shown at the bottom (names begin with “e”).

D, Quantification of enhancer RNA (eRNA) from PRO-seq in the three enhancers around the Cxcl1 gene.

E, Nascent RNA levels of the expressed Chemokine genes shown as the z-cores heatmap of PRO-seq RPM in the three isogenic cells.

F, Quantification of p300 and histone modification levels (left) and p53^R172H occupancy (right) at the promoter and enhancers of the Cxcl1 gene using CUT&RUN. P-values are calculated excluding e8697 using a paired t-test.