Figure 6. p53^R172H occupancy in Cxcl1 Enhancers is dependent on NF-κB.

A, Enriched transcription factor motifs at the promoters and enhancers of the p53^R172H-dependent chemokine genes identified using HOMER¹⁰⁵.

B, NF-κB occupancy at the Cxcl1 gene and enhancers in Trp53^R172H/− and Trp53^−/− cells using CUT&RUN. Non-specific IgG is used as a control. The HOMER-identified NF-κB motifs are shown at the bottom (red bars).

C, Quantification of NF-κB occupancy at the promoter and enhancers of the Cxcl1 gene using CUT&RUN. P-values are calculated excluding e8697 using a paired t-test.

D, Quantification of NF-κB occupancy at the NF-κB CUT&RUN peaks using CUT&RUN.

E, Quantification of NF-κB phosphorylation in the Trp53^R172H/− and Trp53^−/− cells using ELISA on cell extracts. The antibody targets S536 in the p65 subunit of NF-κB.

F, Quantification of NF-κB localization in the cytoplasm vs nucleus using western blot (serial dilution of protein lysates). Vinculin and histone H3 were used as the markers of cytoplasmic and nuclear fractions, respectively. P-values are calculated from a two-way ANOVA test.