Figure 7. NF-κB inhibition abrogates p53^R172H occupancy.

A, Effect of inhibiting NF-κB activation (5 uM TPCA-1) in NF-κB and p53^R172H occupancy at the Cxcl1 gene and enhancers. DMSO treatment serves as a control.

B, Change in p53^R172H occupancy (left) and NF-κB occupancy (right) by NF-κB inhibition in the p53^R172H and NF-κB CUT&RUN peaks, respectively.

C. Effects of TPCA-1 treatment in NF-κB (left) and p53^R172H (right) occupancy in the two detectable enhancers of the Cxcl1 gene.

D, Effects of TPCA-1 treatment in Trp53^R172H (left) and Cxcl1 (right) mRNA levels using RT-qPCR.