Figure 4:

L. reuteri promotes the secretion of enterocytic hormones. A) Gut Cell Atlas annotated UMAP of the adult jejunum (adapted from Danhof et al 2023), highlighting the enteroendocrine marker CHGA (B), the enterocyte marker SI (C), vasopressin (AVP, D), luteinizing hormone subunit beta (LHB, E), and adipolin (C1QTNF12, F). G) Adipolin visualized in human jejunal tissue. Scale bar represents 50 μm. Secretion of H) vasopressin and I) luteinizing hormone subunit beta and J) the lack of secretion of adipolin from whole human jejunal tissue using the method shown in Figure 3A except with ex vivo human jejunal intestinal tissue. Shape represents unique human intestinal donors. Significance was determined using a linear mixed model with p <0.05 considered as significant.