Fig. 3.

(A) Effects of 30 μM of DHS in LF1 cells at different incubation times and (B) at different concentrations for 24 h on SIRT1 protein levels. (C and D) Quantification of SIRT1 protein by densitometric analysis of the Western blot and normalization to the internal loading control (β-actin and vinculin, respectively). Data are the mean ± SD from at least three independent experiments; values are expressed as arbitrary units (a.u.). (**p ≤ 0.01 compared with control cells). (E) Time-dependent analysis of SIRT1transcripts by quantitative real-time PCR (RT-PCR) in LF1 cells treated with 30 μM DHS or RSV at the indicated times. (F) Time dependent analysis of SIRT1 protein levels by Western blot in LF1 cells treated with 30 μM DHS or RSV at the indicated time and (G) the relative densitometric analysis. Both RT-PCR and Western Blot were performed on samples derived from the same experiment.