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Primers sequences used for RT-qPCR.
| Genes | Forward (5′–3′) | Reverse (5′–3′) |
|---|---|---|
| GAPDH | ACAGCAACAGGGTGGTGGAC | TTTGAGGGTGCAGCGAACTT |
| MUC5AC | CAACACACCACTGCAAGAGC | GGCTGTGTGGTAGCTGAAGT |
| MUC5B | CCTACGTGCCGCTCTCTAAG | CAGGCAGGTCAACTTCCCAT |
| EGFR | ACTGCGAGAACCAAGCTACT | GGAGGCTCAGAAAGTGGTCT |
| PI3K | GAACAGGGCAGCTTCAATGC | CTCCTTCTGGGTCCGGAGTA |
| Akt | CCTGGACTACTTGCACTCCG | CACAGCCCGAAGTCCGTTAT |
