Table 7. Analysis of metabolic pathways in fecal samples from the four groups of rats.
| Group | KEGG | CAZy | eggNOG | |||||||
|---|---|---|---|---|---|---|---|---|---|---|
| One carbon pool by folate (PATH: ko00670) | Porphyrin and chlorophyll metabolism (PATH: ko00860) | Selenocompound metabolism (PATH: ko00450) | Cell motility | Glycoside Hydrolase Family 8 | P: Inorganic ion transport and metabolism | E: Amino acid transport and metabolism | K: Transcription | |||
| Probiotic group A (GY) (%) | 3,480.958±339.169 | 3,368.791±242.132 | 1,836.698±161.113 | 70.910±42.239 | 66.071±19.247 | 44,725.499±3,380.265 | 32,495.243±3,715.999 | 71,371.947±5,781.980 | ||
| Probiotic group B (JG) (%) | 3,366.241±235.794 | 3,457.640±369.448 | 1,813.249±114.329 | 91.934±34.663 | 57.848±15.714 | 44,766.557±2,727.302 | 31,956.782±2,508.184 | 75,372.276±4,187.976 | ||
| Control group (KB) (%) | 2,892.185±218.959 | 2,801.323±193.877 | 1,599.867±115.234 | 158.832±27.312 | 38.394±8.563 | 39,973.806±2,078.370 | 27,968.824±1,687.457 | 79,803.788±7,057.325 | ||
| Model group (MX) (%) | 3,245.163±351.657 | 3,375.826±524.712 | 1,836.698±161.113 | 104.494±41.881 | 48.135±15.765 | 42,202.205±2,747.064 | 30,020.183±2,856.570 | 75,193.231±6,510.051 | ||
| t value | ||||||||||
| KB vs. GY | 4.612 | 5.785 | 3.781 | −5.528 | 4.155 | 3.787 | 3.507 | −2.923 | ||
| KB vs. JG | 4.659 | 4.974 | 4.157 | −4.794 | 3.438 | 4.420 | 4.172 | −1.708 | ||
| GY vs. JG | 0.878 | −0.636 | 0.375 | −1.217 | 1.046 | −0.030 | 0.380 | −1.772 | ||
| MX vs. GY | 1.604 | −0.038 | 2.266 | −1.785 | 2.280 | 1.832 | 1.670 | −1.388 | ||
| MX vs. JG | 0.967 | 0.403 | 2.267 | −0.731 | 1.380 | 2.095 | 1.611 | 0.073 | ||
| KB vs. MX | −2.895 | −3.248 | −1.472 | 3.437 | −1.717 | −2.046 | −1.955 | 1.519 | ||
| Adjusted P value | ||||||||||
| KB vs. GY | 0.02 | 0.047 | 0.03 | 0.01 | 0.02 | 0.04 | 0.005 | 0.04 | ||
| KB vs. JG | 0.24 | >0.99 | 0.19 | 0.01 | 0.48 | 0.03 | 0.003 | 0.12 | ||
| GY vs. JG | >0.99 | >0.99 | >0.99 | >0.99 | >0.99 | >0.99 | >0.99 | >0.99 | ||
| MX vs. GY | >0.99 | >0.99 | >0.99 | >0.99 | >0.99 | >0.99 | >0.99 | >0.99 | ||
| MX vs. JG | >0.99 | >0.99 | >0.99 | >0.99 | >0.99 | >0.99 | >0.99 | >0.99 | ||
| KB vs. MX | >0.99 | >0.99 | >0.99 | 0.62 | >0.99 | 0.86 | 0.44 | 0.99 | ||
Data are presented as mean ± standard error of the mean. Control group (KB): fed with water; model group (MX): fed with 5% DSS to induce acute diarrhea; probiotic group A (GY): acute diarrhea model fed with Clostridium butyricum, Bifidobacterium infantis, and Saccharomyces boulardii at the same time; probiotics group B (JG): acute diarrhea model fed with Clostridium butyricum, Bifidobacterium infantis, and Saccharomyces boulardii at 2-hour interval. KEGG, Kyoto Encyclopedia of Genes and Genomes; CAZy, Carbohydrate-Active Enzymes database; eggNOG, evolutionary genealogy of genes: Non-supervised Orthologous Groups.