Figure 2.

Read-through facilitated by p2luc constructs. Each of the 15 sequences listed on the x-axis were tested for their ability to facilitate read-through of Photinus luciferase using the fusion vector p2luc. Sequences 1, 4, 11, 12 and 14 represent exactly the +1 and +2 triplet sequences from groups 1, 3, 4, 2 and 2 respectively. The other 10 sequences were either substitutions of a single nucleotide position (sequences 2, 3 and 5–9) or a substitution of the stop codon (sequences 10, 13 and 15).