Table 2.
Binding affinity of TylF and its variants to macrocin, SAM and SAH by NanoDSF.
| TylF | TylFQ138H, F232Y | TylFT36S, V54A | TylFY139F | TylFL123P, N165S, V182G | TylFV54D | |
|---|---|---|---|---|---|---|
| TmB (°C) | 38.358 | 36.74 | 29.609 | 35.071 | 37.549 | 34.616 |
| TmB+m (°C) | 41.493 | 38.799 | 37.072 | 36.022 | 37.862 | 39.297 |
| TmB+m+SAM | 46.774 | 42.903 | 43.118 | 44.978 | 39.642 | 42.419 |
| TmB+m+SAH (°C) | 46.778 | 44.057 | 41.909 | 41.05 | 45.453 | 45.09 |
| ΔTmm (°C) | 3.135 | 2.059 | 7.463 | 4.681 | 0.951 | 0.313 |
| ΔTmSAM (°C) | 5.281 | 4.104 | 6.046 | 5.681 | 3.62 | 4.557 |
| ΔTmSAH (°C) | 5.285 | 5.258 | 4.837 | 1.753 | 9.431 | 7.228 |
B, buffer; m, macrocin; TmB was calculated in the buffer system and so on, the average of the three duplications on show; ΔTm was calculated by the following formula: ΔTmm = TmB+m -TmB; ΔTmSAM = TmB+m+SAM - TmB+m; ΔTmSAH = TmB+m+SAH -TmB+m.