TABLE 2.
| Gene | Function/pathway | Strain | Technique | Reference |
|---|---|---|---|---|
| aceE | Pyruvate dehydrogenase E1 component | CRE-166, KPN46, Z4160 | INseq | (107) |
| ackAa | Acetate kinase | MH258 | INseq | (195) |
| acrA | Multidrug efflux pump subunit | CRE-166, KPN46, Z4160 | INseq | (107) |
| adhE | Aldehyde-alcohol dehydrogenase | CRE-166, KPN46, Z4160 | INseq | (107) |
| arcBa | Aerobic respiration control sensor protein | MH258, CRE-166, KPN46, Z4160, C3091 | INseq, STM | (107, 193, 195) |
| bamBa | Outer membrane beta-barrel assembly protein | MH258 | INseq | (195) |
| bamE | Outer membrane beta-barrel assembly protein | MH258 | INseq | (195) |
| bglY | Beta-galactosidase | CRE-166, KPN46, Z4160 | INseq | (107) |
| carA | Carbamoyl phosphate synthase small subunit | KPN46, Z4160 | INseq | (107) |
| carB | Carbamoyl phosphate synthase large subunit | CRE-166, Z4160 | INseq | (107) |
| csrDa | Putative lipoprotein | MH258 | INseq | (195) |
| cvpA | Colicin V production protein | CRE-166, KPN46, Z4160 | INseq | (107) |
| cyaAa | Adenylate cyclase | MH258 | INseq | (195) |
| cydA | Cytochrome bd-I ubiquinol oxidase subunit 1 | CRE-166, KPN46, Z4160 | INseq | (107) |
| cyoA | Cytochrome O ubiquinol oxidase subunit II | MH258 | INseq | (195) |
| cyoB | Cytochrome O ubiquinol oxidase subunit I | MH258 | INseq | (195) |
| fnr | Fumarate and nitrate reduction regulatory protein | CRE-166, KPN46, Z4160 | INseq | (107) |
| focA | Formate transporter | CRE-166, KPN46, Z4160 | INseq | (107) |
| gatRa | Galactitol utilization operon repressor | MH258 | INseq | (195) |
| glgP | Alpha-glucan phosphorylase | LM21 | STM | (192) |
| glnA | Glutamine synthetase | CRE-166, KPN46, Z4160 | INseq | (107) |
| gltB | Glutamate synthase (NADPH) large chain | MH258 | INseq | (195) |
| gshAa | Glutamate-cysteine ligase | MH258 | INseq | (195) |
| gshBa | Glutathione synthetase | MH258 | INseq | (195) |
| gvR | Glycine metabolism regulator | LM21 | STM | (192) |
| hemN | Oxygen-independent coproporphyringogen III oxidase | MH258 | INseq | (195) |
| hrcU | Harpin type III secretion system | LM21 | STM | (192) |
| hscAa | Chaperone protein | MH258 | INseq | (195) |
| hscBa | Chaperone protein | MH258 | INseq | (195) |
| hupA | DNA folding | C3091 | STM | (193) |
| lacY | Lactose permease | MH258 | INseq | (195) |
| miaA | tRNA dimethylallyltransferase | CRE-166, KPN46, Z4160 | INseq | (107) |
| mtlD | Mannitol-1-phosphate 5-dehydrogenase | CRE-166, KPN46, Z4160 | INseq | (107) |
| ntrC | Nitrogen metabolism regulator | LM21 | STM | (192) |
| nuoM | NADH-ubiquinone oxidoreductase chain M | MH258 | INseq | (195) |
| ompA | Outer membrane protein | C3091 | STM | (193) |
| ompCa | Outer membrane porin C | MH258, CRE-166, KPN46, Z4160 | INseq | (107, 195) |
| pal | Peptidoglycan-associated lipoprotein | CRE-166, KPN46, Z4160 | INseq | (107) |
| pflA | Pyruvate formate-lyase 1-activating enzyme | CRE-166, KPN46, Z4160 | INseq | (107) |
| pflB | Formate acetyltransferase 1 | CRE-166, KPN46, Z4160 | INseq | (107) |
| pgi | Glucose-6-phosphate isomerase | CRE-166, KPN46, Z4160 | INseq | (107) |
| pheC | Cyclohexadienyl dehydratase | LM21 | STM | (192) |
| plsX | Fatty acid and phospholipid synthesis | C3091 | STM | (193) |
| ppx/gppA | Guanosine-5′-triphosphate, 3′-diphosphate pyrophosphatase | MH258 | INseq | (195) |
| Ptaa | BioD-like N-terminal domain/phosphate acetyltransferase | MH258 | INseq | (195) |
| ptsIa | Phosphoenolpyruvate-protein phosphotransferase | MH258, CRE-166, KPN46, Z4160 | INseq | (107, 195) |
| purC | Phosphoribosylaminoimidazole-succinocarboxamide synthase | CRE-166, KPN46, Z4160 | INseq | (107) |
| purH | Bifunctional purine biosynthesis protein | CRE-166, KPN46, Z4160 | INseq | (107) |
| pyg | Glycogen phosphorylase | MH258 | INseq | (195) |
| pykF | Pyruvate kinase I | CRE-166, KPN46, Z4160 | INseq | (107) |
| rimO | Ribosomal protein S12p Asp88 methylthiotransferase | MH258 | INseq | (195) |
| setA | Sugar efflux transporter A | CRE-166, KPN46, Z4160 | INseq | (107) |
| surA | Folding of outer membrane proteins | C3091 | STM | (193) |
| tamA | Outer membrane component of TAM transport system | MH258 | INseq | (195) |
| tamB | Inner membrane component of TAM transport system | MH258 | INseq | (195) |
| tatA | Sec-independent protein translocase protein | CRE-166, KPN46, Z4160 | INseq | (107) |
| tatC | Sec-independent protein translocase protein | CRE-166, KPN46, Z4160 | INseq | (107) |
| tolA | Tol-Pal system protein | CRE-166, KPN46, Z4160 | INseq | (107) |
| tufA | Protein synthesis—elongation factor | C3091 | STM | (193) |
| typAa | GTP-binding protein | MH258 | INseq | (195) |
| uvrA | Excinuclease ABC subunit A | MH258 | INseq | (195) |
| waaE | LPS core synthesis | C3091 | STM | (193) |
| waaL | LPS core synthesis | C3091 | STM | (193) |
| wbbO | O-antigen synthesis | C3091 | STM | (193) |
| xylA | Xylose isomerase | MH258 | INseq | (195) |
| yeiE | HTH-type transcriptional activator | CRE-166, KPN46, Z4160 | INseq | (107) |
| ygjA | DedA family inner membrane protein | MH258 | INseq | (195) |
| ygjD | O-sialoglycoprotein endo-peptidase | LM21 | STM | (192) |
a
Denotes a gene that was also found to significantly impact growth under in vitro conditions.
b
Summary of genes identified in previous Tn-seq and STM screens as contributing to K. pneumoniae GI colonization.
c
CRE-166: carbapenem-resistant lung isolate, ST258. KPN46: non-epidemic, antibiotic susceptible blood isolates, ST433. Z4160: ESBL-producing blood isolates, ST45. LM21: cutaneous wound isolates, K35. C3091: streptomycin-resistant UTI isolates, K16. MH258: MDR blood isolates, ST258.