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[Preprint]. 2024 Aug 30:rs.3.rs-4844371. [Version 1] doi: 10.21203/rs.3.rs-4844371/v1

Figure 3. Amiloride efficacy is enhanced by EGFR knockdown in HSPC cells and by HER2 knockdown in CRPC cells.

Figure 3

(A) CRPC C4–2 cells were transfected with control (CT) or EGFR or ErbB3 siRNA or (B) HER2 siRNA and treated with or without 75μM amiloride before being analysed for changes in viability with the MTT assay. Error bars represent standard deviation. Experiments were performed in triplicate. (C) Whole cell immunoblot for siRNA efficacy. 20μg of protein were loaded per lane. Tubulin was used as a loading control. Densitometry was performed using ImageJ. Error bars represent standard error of the mean.