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. 2024 Jun 10;5(5):zqae030. doi: 10.1093/function/zqae030

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© The Author(s) 2024. Published by Oxford University Press on behalf of American Physiological Society.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (https://creativecommons.org/licenses/by/4.0/), which permits unrestricted reuse, distribution, and reproduction in any medium, provided the original work is properly cited.

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Confocal imaging shows intracellular calcium concentration before and after PAR1 agonist TFLLR-NH2 application, with peak values of calcium release fitted by Hill’s equation (EC50 = 3.0 ± 0.8 nM). — PAR1 response in cultured HRMCs. (A) Representative confocal imaging of intracellular [Ca²⁺]_i concentration (Fluo-8H, AM fluorescence) before and after acute application of the PAR1 selective agonist TFLLR-NH₂ (50 n m). The scale bar is 100 µm. (B) Peak values of PAR1 [Ca²⁺]_i release (store release, first peak) fitted by Hill’s equation with half maximal effective concentration (EC₅₀) 3.0 ± 0.8 n m.