REAGENT or RESOURCE	SOURCE	IDENTIFIER
Antibodies
anti-NUDT5	Abcam	Cat #ab129172; RRID:AB_11150622
anti-Septin8	Abcam	Cat #ab191404
Anti-GAPDH	Cell signaling technology	Cat #2118; RRID:AB_561053
Bacterial and virus strains
E. coli TOP10 competent cells	GB104	Tiangen
Biological samples
FFPE samples	This paper	PCSHA (Table S1)
FFPE biopsy samples	This paper	PCSHA-biopsy (Table S5)
Chemicals, peptides, and recombinant proteins
Heptane	Sigma-Aldrich	Cat #246654-2L
Ethanol	Sinopharm Chemical Reagent limited corporation	Cat #10009218
Urea	Sigma-Aldrich	Cat #U1250-1KG
Trizma Base	Sigma-Aldrich	Cat #T6791-500G
Formic acid (FA)	Fisher Scientific	Cat #A117-50
HCl	Hualishi	Cat #HLS HCL001C
Thiourea	Sigma-Aldrich	Cat #T8656-500G
Ammonium bicarbonate (ABB)	General-Reagent	Cat #G12990A
Tris (2-carboxyethyl) phosphine (TCEP)	Adamas-beta	Cat #61820E
Iodoacetamide (IAA)	Sigma-Aldrich	Cat #I6125
Trypsin	Hualishi Tech	Cat #HLS TRY001C
Lys-C	Hualishi	Cat #HLS LYS001C
Trifluoroacetic acid (TFA)	Fisher Chemical	Cat #85183
Water	Fisher Chemical	Cat #W6-4
Acetonitrile	Fisher Chemical	Cat #A955-4
Methanol	Sigma-Aldrich	Cat #HLS TRY001C
Acetonitrile	General-Reagent	Cat #G80988B
Xylene	SINOPHARM	10023418
Neutral balsam	SINOPHARM	10004160
Blasticidin S HCl	Beyotime	ST018
Puromycin	Yeasen	60209ES59
Phosphate Buffer Solution	Servicebio	G4202
Matrigel® Matrix	Corning	354234
Crystal Violet Staining Solution	Beyotime	C0121-100mL
Critical commercial assays
BeyoClick™ EdU Proliferation Kit with Alexa Fluor 488	Beyotiome	C0071S
Deposited data
Raw data	This paper	https://www.iprox.org/(IPX0005748000)
Experimental models: Cell lines
PC-3	National Collection of Authenticated Cell Cultures	TCHu158
DU 145	National Collection of Authenticated Cell Cultures	TCHu222
LNCaP	National Collection of Authenticated Cell Cultures	TCHu173
HEK293T	National Collection of Authenticated Cell Cultures	GNHu17
Oligonucleotides
CRISPR-SEPTIN8-gRNA1	This paper	5′- TGAGGAAGCCAGTCACCATG -3′
CRISPR-NUDT5-gRNA1	This paper	5′- GAAACAGTTCCGACCACCAA -3′
CRISPR-SEPTIN8-gRNA2	This paper	5′- ACTGGCTTCCTCAGTCTCGA -3′
CRISPR-NUDT5-gRNA2	This paper	5′- TCACTATGAGTGTATCGTTC -3′
CRISPR-Scramble-gRNA	This paper	5′- ACGGAGGCTAAGCGTCGCAA -3′
Recombinant DNA
Lenti-CRISPR-V2 Vector	Addgene	Addgene Plasmid # 52961
Software and algorithms
Xcalibur	Thermo Fisher Scientific	OPTON-30965
DIA-NN version 1.8.0-linux	Demichev et al.53	https://github.com/vdemichev/DiaNN
R version 4.0.2	R Project	https://www.r-project.org
Metascape	Zhou et al., 201932	https://metascape.org/gp/index.html#/main/step1
Other
SOLAμ	Thermo Fisher Scientific	Cat # 62209-001