Figure 2.

The gut microbiota regulated ovarian toxicity induced by cisplatin

(A) Ovarian weight of Ctrl, Cis, Cis+Ctrl-FMT, and Cis+Cis-FMT mice (n = 6).

(B) Serum levels of E2 and FSH in Ctrl, Cis, Cis+Ctrl-FMT, and Cis+Cis-FMT mice (n = 6).

(C) Quantification of primordial, primary + secondary, antral, and atretic follicles (n = 6).

(D) Representative images of ovaries and antral follicles in recipient mice by H&E.

(E) Ovarian weight of Ctrl, Cis-POI, and Cis-POI+ L. reuteri mice (n = 6).

(F) Serum levels of E2 and FSH in Ctrl, Cis-POI, and Cis-POI+ L. reuteri group (n = 6).

(G) H&E staining of ovaries from mice in each group.

(H) Quantitative analysis of the primordial, primary + secondary, antral, and atretic follicles (n = 6).

(I) Representative images of H&E-stained histological sections from antral follicles and TUNEL-based quantification of the apoptotic index in the antral follicles (n = 17 for Ctrl, n = 21 for Cis-POI, and n = 23 for Cis-POI+L. reuteri). The data are all presented as the mean ± SEM. ∗p < 0.05, ∗∗p < 0.01, ∗∗∗p < 0.001; one-way ANOVA following the Benjamini and Hochberg’s method for multiple adjustments in (A, B, and C); one-way ANOVA following the Dunnett’s multiple comparisons test in (F, G, I, and J).