Figure 6.

Wig-1 protein produced in mammalian cells also specifically binds dsRNA. (A) NIH 3T3 cells were transfected with the FLAG-tagged mouse wig-1 expression plasmid. After 48 h total protein cell lysates were prepared and 50 µg aliquots were incubated with poly(I·C) immobilized on agarose beads. The incubations were performed in the absence or presence of the competitor poly(C) or poly(I·C). The bound wig-1 protein was analyzed by immunoblotting using anti-FLAG antibodies. (B) As in (A) except that NIH 3T3 cells were transfected with a FLAG-tagged wig-1 expression plasmid lacking the first zinc finger domain.