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. 2024 Sep 10;9(17):e171659. doi: 10.1172/jci.insight.171659

Figure 8. Dexamethasone does not alter intracellular arginase-1 expression but dampens arginase-1 release from healthy neutrophils.

Figure 8

Fresh neutrophils were isolated from healthy controls and pretreated with varying concentrations of dexamethasone (0, 0.1 μM, 1 μM, 10 μM) for 4 hours. Cells were left unstimulated or stimulated with 5 μg/mL fMLP or 200 ng/mL IL-8. Geometric MFI (gMFI) ratio of (A) intracellular arginase-1 expression (n = 5) and (B) surface CXCR2 expression (n = 5) measured using traditional flow cytometry. (C) MPO release (n = 6; n = 2 with dexamethasone conc. 33 μM and 100 μM) and (D) arginase-1 activity (n = 6) measured in supernatants using ELISA and arginase-1 enzyme assay, respectively. Statistical analysis was performed using repeated measures 2-way ANOVA in A, B, and D. C was analyzed using mixed effects analysis. P values represent the effect of dexamethasone concentration, *P < 0.05. Median with IQR is shown.