Figure 1.

Two-hybrid screen for spTAF72-interacting protein. (A) The bait plasmid (upper) encodes a Gal4 BD fusion of the C-terminal portion of spTAF72, which contains WD-repeat motifs. An isolated clone (lower) encodes a Gal4 AD fusion of a nearly entire region of a predicted protein, SPCC16C4.18c (referred to as spTAF50), which has homology to histone H4-like TAFs. (B) AH109 cells carrying the indicated pairs of plasmids were spotted onto synthetic complete medium lacking tryptophan, leucine, histidine and adenine and allowed to grow at 30°C for 2 days. Growth on this medium implies interaction. The cells carrying the BD-TAF72 and AD-TAF50 plasmids (lower right) also showed a Mel⁺ phenotype as judged by blue color on plates containing 5-bromo-4-chloro-3-indolyl-α-d-galactopyranoside (data not shown).