Table 1. Yeast strains.
| Strain | Genotype | Source |
|---|---|---|
| S.cerevisiae | ||
| AH109 | MATa trp1-901 leu2-3,112 ura3-52 his3-200 gal4Δ gal80Δ | Clontech |
| LYS2::GAL1-HIS3 GAL2-ADE2 URA3::MEL1-lacZ | ||
| S.pombe | ||
| JY741 | h– ade6-M216 leu1 ura4-D18 | M. Yamamoto |
| JY746 | h+ ade6-M210 leu1 ura4-D18 | M. Yamamoto |
| HMP10 | h–/h+ ade6-M216/ade6-M210 leu1/leu1 ura4-D18/ura4-D18 | (31) |
| HMP47 | h– ade6-M216 leu1 ura4-D18 gcn53xHA–ura4+ | (16) |
| HMP48 | h– ade6-M216 leu1 ura4-D18 gcn53xHA–ura4+ taf73FLAG | (16) |
| HMP49 | h– ade6-M216 leu1 ura4-D18 gcn53xHA–ura4+ taf72FLAG | (16) |
| HMP54 | h– ade6-M216 leu1 ura4-D18 gcn53xHA | This study |
| HMP55 | h– ade6-M216 leu1 ura4-D18 gcn53xHA taf73FLAG | This study |
| HMP56 | h– ade6-M216 leu1 ura4-D18 gcn53xHA taf72FLAG | This study |
| HMP57 | h– ade6-M216 leu1 ura4-D18 taf50Myc–ura4+ | This study |
| HMP58 | h– ade6-M216 leu1 ura4-D18 gcn53xHA taf50Myc–ura4+ | This study |
| HMP59 | h– ade6-M216 leu1 ura4-D18 gcn53xHA taf73FLAG taf50Myc–ura4+ | This study |
| HMP60 | h– ade6-M216 leu1 ura4-D18 gcn53xHA taf72FLAG taf50Myc–ura4+ | This study |
HMP10 is a diploid constructed by a cross between JY741 and JY746 and all the other HMP strains derived from JY741.