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. 2024 Sep 10;9(17):e179291. doi: 10.1172/jci.insight.179291

Figure 2. Paternal high-cholesterol diet feeding does not affect body weight or serum lipid levels in F1 offspring.

Figure 2

Three-week-old male LDLR–/– mice were fed an LCD or HCD diet for 8 weeks before mating with control female LDLR–/– mice. Three-week-old F1 offspring were fed an LCD for 16 weeks and euthanized at 19 weeks of age. (A) Birth weight (day 0) and body weight of F1 pups before weaning (n = 5; 2-way ANOVA followed by Bonferroni’s multiple-comparison test). (B and C) Growth curves of male (B) and female (C) F1 offspring were measured (n = 9–14; 2-way ANOVA followed by Bonferroni’s multiple-comparison test). (D and E) Lean and fat mass were measure in male (D) and female (E) F1 offspring (n = 9–11, 2-sample, 2-tailed Student’s t test). (F and G) Serum cholesterol and triglyceride levels were measured in male and female offspring (n = 9–10, 2-sample, 2-tailed Student’s t test). (H and I) Serum lipoprotein fractions (VLDL-C, LDL-C, and HDL-C) were isolated from male (H) and female (I) F1 offspring and cholesterol levels from each fraction were measured (n = 7–9, 2-sample, 2-tailed Student’s t test). VLDL-C, very low-density lipoprotein cholesterol; LDL-C, low density lipoprotein cholesterol; HDL-C, high density lipoprotein cholesterol. All data are plotted as mean ± SEM.