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. 2024 Sep 10;9(17):e179291. doi: 10.1172/jci.insight.179291

Figure 5. Paternal hypercholesterolemia elicits transcriptomic changes in the intima of F1 LDL receptor–deficient mice.

Figure 5

Three-week-old male LDLR–/– mice were fed an LCD or HCD diet for 8 weeks before mating with control female LDLR–/– mice. Three-week-old F1 descendants were fed a LCD for 16 weeks. Total RNAs were isolated from the intima of F1 offspring and used for RNA-Seq analysis. (A and B) Volcano plots of differentially expressed genes (DEGs) in the intima of male offspring (A) and female offspring (B) from HCD-fed LDLR–/– sires. Colored dots represent the enriched (red dots) or depleted (blue dots) DEGs with a FDR of < 0.1 and a FC > 1.5 as a cut-off threshold. (C and D) GOBP terms significantly associated with upregulated DEGs in intima of male offspring (C) and female offspring (D) from HCD-fed sires. The P values were computed by the modified Fisher’s exact test using the DAVID bioinformatics tool. The vertical dash line indicates the significance level of α = 0.05. The y axis displays the GOBP terms, while the x axis displays the P values (n = 4–7 each group).