Figure 6. Paternal hypercholesterolemia alters atherosclerosis-related gene expression in the intima of female F1 offspring.

Three-week-old male LDLR^–/– mice were fed an LCD or HCD diet for 8 weeks before mating with control female LDLR^–/– mice. Three-week-old F1 descendants were fed a LCD for 16 weeks. Total RNAs were isolated from the intima of F1 offspring and used for RNA-Seq analysis. (A) Gene set scores of the prioritized GOBP terms of male and female offspring from LCD or HCD-fed sires. The gene set score was calculated using the FAIME algorithm. (B) Heatmap representation of DEGs involved in the indicated GOBP terms. Each column shows 1 individual gene, and each row shows a biological replicate of mouse. Red represents relatively increased gene expression, whereas blue denotes downregulation (n = 4–7 each group).