Fig. 1.

Bilateral carotid artery stenosis (BCAS) in mice induces sustained cerebral hypoperfusion. A Illustration of BCAS model modified from the paper by Ref Eun et al. [28]. B Experimental protocol. Mice underwent Sham or BCAS surgery. Arrowheads: indicate the time points at which the cerebral blood flow (CBF) or immunostaining (IF) were performed. C Representative periCam PSI images by a two-dimensional laser speckle contrast analysis system (PSI analysis) of regional CBF (rCBF) in male C57/B6J mice before, 10 min, 3 days, 7 days, 14 days, and 28 days after Sham or BCAS surgery. Dashedcircle: region of interest (ROI). D–F Quantification analysis of rCBF. Data are expressed as mean ± SD. n = 5–8 per group. *p < 0.05, **p < 0.01 vs. Sham. One Sample t-test