Fig. 1. Workflow overview: generating an integrated regulatory interaction map of RNA-binding proteins.

A–C The results of BioID2, Perturb-seq, and publicly available ENCODE eCLIP assays were independently processed and normalized across RBPs. D The resulting Z-scores were used to estimate the cosine distance between all pairs of the tested RBPs and to calculate empirical left-tailed p-values for RBP-RBP similarities. For each pair of RBPs, the p-values from three assays were aggregated as in ref. ¹¹⁷ to obtain a single measure of similarity between RBPs across the feature spaces from the three modalities. The resulting matrix of pairwise similarities was defined as the Integrated Regulatory Interaction Map (IRIM) that simultaneously captures physical and functional interactions between RBPs.