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Mitochondrial transcription in mtDNA-depleted cells. Northern analysis of mtDNA-encoded ND2 mRNA (indicated with an arrow) and nucleus-encoded β-actin mRNA (indicated with an arrowhead) during mtDNA depletion–repletion. A second faster-migrating RNA species (presumably a truncated ND2 transcript) was always observed in the presence of EB (most obvious in lane 3D). Time points during mtDNA depletion–repletion are indicated in the same manner as in Figure 1.
