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. 2024 Aug 29;137(16):jcs262202. doi: 10.1242/jcs.262202

Fig. 1.

Fig. 1.

Protocell engulfment by wound macrophages. (A) Schematic of denatured-BSA-containing FITC-labelled proteinosome-based protocells (López-Cuevas et al., 2022). (B) Schematic for experiments in C and D showing wounds, injection site and imaging region (blue box). (Ci) Confocal images from a time-lapse movie of a wounded Tg(mpeg1:mCherry) larva after FITC-protocell injection, showing protocells at the wound region, and within intersegmental vessels (white dotted lines) or macrophages (white arrowheads) at 0.5, 2 and 7 hpw. (Cii) Confocal images of the same experiment at 24 and 48 hpw. (D) Graph showing percentage of macrophages containing protocell(s) for wounded fish treated with a single (W+PCs) or double (PCs+W+PCs) protocell dose. White dashed box in C indicates wound site; each dot in D represents the mean of all fish analysed. Error bars are mean±s.e.m. *P<0.05; ****P<0.0001 (unpaired two-sided Mann–Whitney test). ISV, intersegmental vessel; Mφ, macrophages; n, number of fish; W, wound. Scale bar: 50 μm.