Table 1. ANOVA of CV of inter-assay variation of MT and GAPDH CPs determined in rat liver by real-time RT–PCR started either with 8.25 (1–3), 25 (4–6) or 75 ng (7–9) cDNA per capillary.

	Control		Sample
	Mean CP	CV (%)	Mean CP	CV (%)
MT 1–3 (8.25 ng cDNA)a	24.559	7.87	31.167	8.77
	24.563	7.94	31.167	7.85
	22.669	3.82	28.842	6.43
MT 4–6 (25 ng cDNA)a	25.884	10.03	32.612	5.29
	21.520	5.85	27.862	6.31
	20.464	5.84	26.642	3.43
MT 7–9 (75 ng cDNA)a	20.801	8.76	27.397	3.63
	22.340	4.48	28.955	3.67
	20.794	8.74	27.397	2.43
GAPDH 1–3 (8.25 ng cDNA)b	23.957	4.58	23.537	4.15
	23.342	6.85	23.432	2.52
	23.350	1.59	22.235	4.33
GAPDH 4–6 (25 ng cDNA)b	22.530	7.94	22.350	3.09
	22.899	10.42	21.730	12.89
	21.801	6.35	20.758	5.27
GAPDH 7–9 (75 ng cDNA)b	19.510	8.56	19.023	7.45
	21.314	9.23	20.525	7.16
	20.279	11.19	19.307	7.86

Given are the mean CP and the CV, each one based on n = 7 for control and n = 6 for sample, respectively.

^aANOVA: testing for differences of CV values, neither control nor sample, nor concentration, nor interaction showed a significant effect.

^bANOVA: for GAPDH there was a significant effect (P < 0.05) for concentration.