Figure 5.

Localization analysis of GFNPs in pancreatic cancer metastasize to liver cancer murine model. (A) Photographs and fluorescence imaging of tissue in vitro of three models of pancreatic cancer metastasize to liver cancer murine derived from Pan02 cells (Ex474 nm/Em525 nm). (B and C) Fluorescence intensity of the heart, liver, spleen, lung, kidney and pancreas between sham group and tumor model group. n=5. (D)HE and immunofluorescence staining results of adjacent tissue section derived from liver cancer tissue. Blue-GFNPs; red-PI; pink-Ki67. Scanning scale bar=2 mm. Detailed scale bar=200 µm. (E) Immunofluorescence and HE staining results of adjacent locations scan of a lung tissue section from Pan02 cell-derived pancreatic metastases tumor model. Blue-GFNPs; red-PI; pink-SMA. Scanning scale bar=2 mm. Detailed scale bar=200 µm. (F) Immunofluorescence and HE staining results of adjacent locations scan of a pancreatic tissue section from Pan02 cell-derived pancreatic metastases tumor model. Blue-GFNPs; red-PI; pink-Ki67.Scanning scale bar=2 mm. Partial scale bar=200 µm. (G) Percent positive cell count of Ki67 at pancreatic tissue. (H) Percent positive cell count of SMA+ cells at pancreatic tissue.

Notes: Datas were exhibited as the Mean ± SEM. * p < 0.05.