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Effect of pH on the fluorescence melting profiles for triplex-forming oligo 4. pH 5.0 (red), pH 6.0 (green), pH 7.0 (black), pH 8.0 (grey) and pH 9.0 (blue). The left panel shows plots of fluorescence against temperature (°C), while the right panel shows the first derivative of the melting profile. The fluorescence is expressed in arbitrary units.
