Table 3. Effect of heating and cooling rates on the fluorescence melting curves obtained with the intramolecular triplex formed by oligo 4.
Rate of heating (°C s–1) | Transition 1 | Transition 2 | |||
---|---|---|---|---|---|
Tm (°C) | ΔH (kJ mol–1) | Tm (°C) | ΔH (kJ mol–1) | ||
0.1 | Melt | 36.0 | 157 | 62.0 | 360 |
Anneal | 36.0 | 149 | 62.1 | 338 | |
0.2 | Melt | 37.9 | 170 | 63.8 | 378 |
Anneal | 37.4 | 167 | 63.7 | 355 | |
0.5 | Melt | 38.8 | 169 | 64.3 | 372 |
Anneal | 36.6 | 163 | 63.3 | 380 | |
1.0 | Melt | 39.8 | 168 | 64.9 | 413 |
Anneal | 36.7 | 176 | 62.3 | 419 |