Table 4. Effect of naphthylquinoline on the fluorescent melting curves of intramolecular DNA triplexes and duplexes.
| Naphthylquinoline (µM) | Triplex | Duplex | ||||
|---|---|---|---|---|---|---|
| Transition 1 | Transition 2 | |||||
| Tm (°C) | ΔH (kJ mol–1) | Tm (°C) | ΔH (kJ mol–1) | Tm (°C) | ΔH (kJ mol–1) | |
| Control | 35.8 | 154 | 61.6 | 374 | 75.4 | 352 |
| 0.1 | 36.8 | 139 | 61.9 | 415 | 75.1 | 353 |
| 0.5 | 45.4 | 126 | 62.6 | 413 | 75.3 | 356 |
| 1 | 48.8 | 139 | 63.7 | 430 | 75.4 | 360 |
| 2 | 53.0 | 149 | 65.0 | 429 | 75.9 | 364 |
| 5 | 59.4 | 149 | 67.3 | 394 | 77.4 | 387 |
| 10 | 63.5 | 149 | 69.5 | 396 | 79.3 | 432 |
The Tm and ΔH values were obtained from van’t Hoff analysis of the melting curves. For the intramolecular triplex the first transition corresponds to the triplex→duplex melt.