Table 5. Thermodynamic parameters obtained for the fluorescence melting curves for intramolecular (oligo 11) and intermolecular (oligo 12) quadruplexes, measured in 50 mM potassium phosphate pH 7.4.
| Tm (°C) | ΔH (kJ mol–1) | |
|---|---|---|
| Intramolecular melt | 71.4 | 252 |
| Intramolecular anneal | 69.2 | 253 |
| Intramolecular slow anneal | 69.0 | 231 |
| Intermolecular melt | 61.2 | 135 |
| Intermolecular anneal | 46.0 | 61.2 |
The Tm and ΔH values were obtained from van’t Hoff analysis of the melting profiles using both the annealing and melting curves. The data for the slow anneal were obtained by increasing the temperature in 1°C steps and leaving the mixture to equilibrate for 1 min before recording the fluorescence.