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. 2000 Nov 15;28(22):4467–4473. doi: 10.1093/nar/28.22.4467

Figure 4.

Figure 4

Analysis of the ability of stable subfragments of U3 and U14 to undergo 5′-cap hypermethylation. Nuclear RNAs were recovered from oocytes 4 h (U3 3′ Domain, U3 Subfragment BC and U14ΔAV) or 8 h (U3 Subfragment C′D) after nuclear injection and analyzed by immunoprecipitation. Diagrams of the U3 3′-domain fragment (A), U3 subfragment BC (B), U3 subfragment C′D (C) and U14ΔAV subfragment (D) are shown above the corresponding cap hypermethylation results (see Materials and Methds for descriptions of fragments). In each panel the subfragment is marked with an asterisk (*). Wild-type U3 served as an internal positive control in (A)–(D). Wild-type U1 was included in (A) while a variant U1 RNA (U1sm– RNA), lacking a functional Sm binding site and unable to undergo cap hypermethylation, was included in (B)–(D).