Figure 1.

One-hybrid and complementation assays of Gal4BD–Ino4p deletions. (A) Yeast strain Y190 was transformed with the Gal4BD–Ino4p protein deletions. Yeast extracts prepared from transformants that were grown in Trp^– synthetic medium were used to conduct β-galactosidase assays. The Ino4p protein deletions were also transformed into an ino4Δ strain and plated on Trp^– synthetic medium. Cells were replica plated onto Trp^– synthetic medium lacking inositol and scored for their ability to complement inositol auxotrophy. (B) Representative plate assay showing ino4Δ complementation by pGBT9-INO4_2–151 and pGBT9-INO4_22–135.