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Two-hybrid assay demonstrating interactions between Gal4BD–Ino4p and other yeast bHLH proteins. Y190 was transformed with pGBT9-INO42–151 and pGAD-bHLH. Extracts were prepared from these strains and assayed for β-galactosidase activity. Values represent the average of at least three separate trials. The location of the bHLH domain is shown (filled box).
